Lab Research Focus
I am interested in the regulation of immune responses by T cells and the differentiation of other bone marrow derived cellular lineages. My research program has both basic animal studies and clinical components. The animal studies focus on several murine models of T cell development and T cell mediated inflammatory disease. One major focus is the analysis of cytokine expression mechanisms and antigen specificity of T cells in allograft rejection. The primary model for these studies is hetrotopic murine cardiac allografts. Studies of T cell development focus on the mechanism(s) of selection of the cytokine phenotype repertoire using a peptide specific TCR transgenic mouse model. I also have a large program in studies of HIV disease, using basic in vitro studies of cell cultures, studies from tissue biopsies and blood cells from patients in HIV treatment clinical trials, and studies of SHIV dynamics in rhesus macaques. In all of these systems, multiple techniques are used including in situ hybridization analysis of viral and cellular RNA species, quantitative-competitive RT-PCR, flow cytometry, immunohistochemistry, and cell culture techniques.
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