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Hybrid Structural Techniques in Cellular and Molecular Biology
Advanced Course MIC 774 - Fall Semester 2008
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Coursemasters: Terje Dokland and Mark Walter
General comments: This course provides an introduction to structural biology with focus on X-ray crystallography, Cryo-Electron microscopy and Image Reconstruction, NMR, and Mass Spectrometry as applied to biological systems. Each component will be taught separately by Drs. Dokland, Walter, Saad, and Prevelige, and will concentrate on providing the student with a basic understanding of the essential principals of the technique as well as its practical applications. Examples will generally be drawn from virology and immunology.
Class Schedule: Every Wednesday and Friday 10:00 - 11:30 AM until December 12, 2008 in CFAR Conference Room (BBRB 256).
Prerequisites: Successful completion of the first year CMB curriculum.
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Day
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Date
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Title |
Instructor |
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Wednesday
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08/20/08 |
Introduction to Structural Biology |
Dokland |
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Friday
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08/22/08 |
Fundamentals of Electron Microscopy |
Dokland |
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Wednesday
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08/27/08 |
Contrast and Image formation in the EM |
Dokland |
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Friday
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08/29/08 |
Phase Contrast - [Class Assignment] |
Dokland |
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Wednesday
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09/03/08 |
Sample Preparation for TEM |
Dokland |
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Friday
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09/05/08 |
3D reconstruction and the Fourier Transform |
Dokland |
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Wednesday
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09/10/08 |
3D reconstruction and tomography |
Dokland |
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Friday
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09/12/08 |
Single-particle reconstruction with EMAN
[Class Assignment] - [Practical Project Assignment] |
Dokland |
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Wednesday
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09/17/08 |
Protein crystallization and crystals
Link to Dr. Walter's lectures for class, (click here) |
Walter |
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Friday
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09/19/08 |
Crystals and the X-ray diffraction experiment |
Walter |
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Tuesday
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09/23/08
12:00 |
Biophysics of virus assembly
BBRB 170 |
George Thomas
UMKC |
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Wednesday
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09/24/08 |
Intensities to electron density maps- Phasing 1 |
Walter |
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Friday
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09/26/08 |
Intensities to electron density maps- Phasing 2 |
Walter |
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Tuesday
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09/30/08
12:00 |
HIV assembly
BBRB 170 |
Paul Spearman
Emory |
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Wednesday
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10/01/08 |
Modeling building and refinement |
Walter |
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Friday
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10/03/08 |
Evaluating protein crystal structures |
Walter |
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Tuesday
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10/07/08
12:00 |
Electron cryotomography of bacteria and viruses
BBRB 170 |
Grant Jensen
Caltech |
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Wednesday
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10/08/08 |
Basic NMR theory |
Saad |
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Friday
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10/10/08 |
Basic NMR theory |
Saad |
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Wednesday
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10/15/08 |
Spin Relaxation and 1D NMR - [Class Assignment] |
Saad |
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Friday
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10/17/08 |
2D NMR |
Saad |
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Wednesday
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10/22/08 |
Protein NMR I |
Saad |
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Friday
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10/24/08 |
Protein NMR II |
Saad |
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Tuesday
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10/28/08
12:00 |
Electron microscopy and virus structure
BBRB 170 |
James Conway
U. Pittsburgy |
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Wednesday
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10/29/08 |
4D NMR Structure Calculations |
Saad |
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Friday
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10/31/08 |
Dynamic NMR - Applications - [Class Assignment] |
Saad |
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Wednesday
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11/05/08 |
Principles of Mass Spectrometry |
Prevelige |
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Friday
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11/07/08 |
Native Mass Spectrometry |
Prevelige |
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Wednesday
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11/12/08 |
Hydrogen/Deuterium Exchange Spectrometry |
Prevelige |
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Friday
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11/14/08 |
Crosslinking and Surface Modification |
Prevelige |
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Wednesday
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11/19/08 |
Principles and Practice of Analytical Ultracentrifugation |
Prevelige |
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Friday
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11/21/08 |
Hybrid methods: putting it all together |
Walter |
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Wednesday
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11/26/08 |
Presentations |
Students |
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Thursday
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11/27/08 |
Thanksgiving |
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Wednesday
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12/03/08 |
Presentations |
Students |
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Friday
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12/05/08 |
Presentations |
Students |
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Wednesday
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12/10/08 |
Presentations |
Students |
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Friday
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12/12/08 |
Presentations |
Students |
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